Coding

Part:BBa_K3468078

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)


PETase N37D

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from N to D at 37 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

There is new salt bridge and hydrogen bond increased between N37D and R267. Salt bridge is a kind of electric interaction.

We use FoldX to judge the thermal stability of N37D and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out whether there is other interactions to make it become more stable.

Fig.1 N37D in PyMOL
[edit]
Categories
Parameters
None